By Jeffrey H. Miller
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Additional info for An Introduction to Genetic Analysis
Crosses of plants differing in one character Figure 2-3 One of the techniques of artificial cross-pollination, demonstrated with the Mimulus guttatus, the yellow monkey flower. To transfer pollen, the experimenter touches anthers from the male parent to the stigma of an emasculated flower, which acts as the female parent. ] or experimenter can cross any two pea plants at will. The anthers from one plant are removed before they have shed their pollen, preventing selfing. Pollen from the other plant is then transferred to the receptive stigma with a paintbrush or on anthers themselves (Figure 2-3).
In regard to this gene, people with normal stature are genotypically d/d, and the dwarf phenotype in principle could be D/d or D/D. However, it is believed that the two “doses” of the D allele in the D/D genotype produce such a severe effect that this genotype is lethal. If this is true, all dwarf individuals are heterozygotes. In pedigree analysis, the main clues for identifying an autosomal dominant disorder with Mendelian inheritance are that the phenotype tends to appear in every generation of the pedigree and that affected fathers and mothers transmit the phenotype to both sons and daughters.
We must be careful about the wording of the hypothesis, because often there are tacit assumptions buried within it. ” We will investigate allele effects on viability in Chapter 6, but for the time being we must keep them in mind as a possible complication because differences in survival would affect the sizes of the various classes. The problem is that if we reject a hypothesis that has hidden components, we do not know which of the components we are rejecting. 4. The outcome of the chi-square test is heavily dependent on sample sizes (numbers in the classes).
An Introduction to Genetic Analysis by Jeffrey H. Miller